SNP-Based Differentiation of Phytophthora infestans Clonal Lineages Using Locked Nucleic Acid Probes and High-Resolution Melt Analysis
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چکیده
منابع مشابه
Infection Efficiency of Four Phytophthora infestans Clonal Lineages and DNA-Based Quantification of Sporangia
The presence and abundance of pathogen inoculum is with host resistance and environmental conditions a key factor in epidemic development. Therefore, several spore-sampling devices have been proposed to monitor pathogen inoculum above fields. However, to make spore sampling more reliable as a management tool and to facilitate its adoption, information on infection efficiency and molecular tools...
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Prior work has shown that the inheritance of resistance to metalaxyl, an oomycete-specific fungicide, is complex and may involve multiple genes. Recent research indicated that a single nucleotide polymorphism (SNP) in the gene encoding RPA190, the largest subunit of RNA polymerase I, confers resistance to metalaxyl (or mefenoxam) in some isolates of the potato late blight pathogen Phytophthora ...
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The Columbia Basin of south-central Washington and north-central Oregon is a major potato-growing region in North America where more than 65,000 ha of potatoes are grown annually. The environment is semiarid (mean rainfall is between 15 and 22.5 cm), and the potato crop is irrigated mostly by center-pivot systems. Although the Columbia Basin is semiarid, potato production is annually threatened...
متن کاملLocked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR.
With an increased emphasis on genotyping of single nucleotide polymorphisms (SNPs) in disease association studies, the genotyping platform of choice is constantly evolving. In addition, the development of more specific SNP assays and appropriate genotype validation applications is becoming increasingly critical to elucidate ambiguous genotypes. In this study, we have used SNP specific Locked Nu...
متن کاملPhenotypic Characterization of Recent Clonal Lineages of Phytophthora infestans in the United States
Danies, G., Small, I. M., Myers, K., Childers, R., and Fry, W. E. 2013. Phenotypic characterization of recent clonal lineages of Phytophthora infestans in the United States. Plant Dis. 97:873-881. Phytophthora infestans, the causal agent of late blight disease, has been reported in the United States and Canada since the mid-nineteenth century. Due to the lack of or very limited sexual reproduct...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2016
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-11-15-1247-re